Isolation and Purification of Planctomycetes associated with Harbor and Lagoon Seagrasses of the Red Sea

  • Holly Bream

Student thesis: Master's Thesis

Abstract

Planctomycetes are members of a unique superphylum along with Verrucomicrobia and Chlamydia, situated in the domain Bacteria. They have distinct structural and morphological features, and discoveries made through phylogenetic analysis indicate their important role in nutrient cycling. Their known relationships with marine photosynthetic organisms led to the formation of this study's hypothesis, namely, that Planctomycetes can be isolated from the biofilm of seagrass species of the Red Sea using cultivation techniques adapted for these organisms. Preparation of solid and liquid media using M13 with both agar and gellan, and 2216 Difco Marine Broth full-­ strength, 1/10-­strength, and with antibiotics, resulted in the successful isolation of Planctomycetes as confirmed by morphological examination and transmission electron microscopy. The work performed in this study provides a solid foundation for further studies to elucidate the metabolic pathways and ecological significance of Planctomycetes.
Date of AwardJul 2012
Original languageEnglish (US)
Awarding Institution
  • Biological, Environmental Science and Engineering
SupervisorVladimir Bajic (Supervisor)

Keywords

  • Planctomycetes
  • Cultivation
  • Purification
  • Seagrass

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