Biocatalysis is considered a green and environmentally friendly technology. Therefore,
novel enzymes and enzymatic systems, together with cascades and protein engineering
approaches, are in high demand.
Here, three very different biocatalytic approaches have been studied. First, the richness
of enzymes in the Red Sea brine pools has been assessed, and the discovery and
characterization of a novel halophilic γ-carbonic anhydrase is described, together with
the protein engineering approach, which boosted the initial catalytic activity of the γ-
carbonic anhydrase. The understanding of polyextremophilicity principles from enzymes
from the Red Sea brine pool, contributes to the bioengineering effort of turning
mesophilic enzymes into more stable variants.
Next, focus is given to the use of amine-transaminases in cascades for chiral amine
synthesis. This resulted in the development of a self-sufficient sustainable cascade for
chiral and non-chiral amine synthesis. This cascade was achieved by combining a lysine
decarboxylase with an amine-transaminase to generate a cheap amino donor source for
a more sustainable reaction economy.
Finally, gas vesicle nanoparticles are functionalized by various engineering principles to
create floating platforms for the immobilization of enzymes. The proof-of-concept was
achieved by anchoring a phytase via anchoring peptides on the gas vesicle
nanoparticles surface. These bioengineering approaches contributed to the effort of
generating first principles for protein engineering.
|Date of Award||Sep 2021|
|Original language||English (US)|
- Biological, Environmental Science and Engineering
|Supervisor||Magnus Rueping (Supervisor)|
- Extremozymes, bioctalysis, cascades, amine-transaminases, Red Sea, Biotransformation