Waves of early transcriptional activation and pluripotency program initiation during human preimplantation development

Rita Vassena, Stéphanie Boué, Eva González-Roca, Begoña Aran, Herbert Auer, Anna Veiga, Juan Carlos Izpisua Belmonte

Research output: Contribution to journalArticlepeer-review

165 Scopus citations

Abstract

The events regulating human preimplantation development are still largely unknown owing to a scarcity of material, ethical and legal limitations and a lack of reliable techniques to faithfully amplify the transcriptome of a single cell. Nonetheless, human embryology is gathering renewed interest due to its close relationship with both stem cell biology and epigenetic reprogramming to pluripotency and their importance in regenerative medicine. Carefully timed genome-wide transcript analyses of single oocytes and embryos uncovered a series of successive waves of embryonic transcriptional initiation that start as early as the 2-cell stage. In addition, we identified the hierarchical activation of genes involved in the regulation of pluripotency. Finally, we developed HumER, a database of human preimplantation gene expression, to serve the scientific community. Importantly, our work links early transcription in the human embryo with the correct execution of the pluripotency program later in development and paves the way for the identification of factors to improve epigenetic reprogramming.

Original languageEnglish (US)
Pages (from-to)3699-3709
Number of pages11
JournalDevelopment
Volume138
Issue number17
DOIs
StatePublished - Sep 1 2011
Externally publishedYes

Keywords

  • Genome activation
  • Human embryos
  • Pluripotency

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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