Validation of microsatellite multiplexes for parentage analysis in a coral reef fish (Lutjanus carponotatus, Lutjanidae)

Hugo B. Harrison, Kevin Andrew Feldheim, Geoffrey P. Jones, Hicham Mansour, Sadhasivam Perumal, David H. Williamson, Michael L. Berumen

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Parentage analysis is an important tool for identifying connectivity patterns in coral reef fishes, but often requires numerous highly polymorphic markers. We isolated 21 polymorphic microsatellite markers from the stripey snapper, Lutjanus carponotatus and describe their integration into three multiplex PCRs. All markers were highly polymorphic with a mean of 24.9 ± 1.8 SE alleles per locus and an average observed heterozygosity of 0.797 ± 0.038 SE across 285 genotyped individuals. Using a simulated dataset, we conclude that the complete marker set provides sufficient resolution to resolve parent–offspring relationships in natural populations with 99.6 ± 0.1 % accuracy in parentage assignments. This multiplex assay provides an effective means of investigating larval dispersal and population connectivity in this fishery-targeted coral reef fish species and informing the design of marine protected area networks for biodiversity conservation and fisheries management.
Original languageEnglish (US)
Pages (from-to)803-806
Number of pages4
JournalConservation Genetics Resources
Volume6
Issue number4
DOIs
StatePublished - May 25 2014

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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