UV and cold tolerance of a pigment-producing Antarctic Janthinobacterium sp. Ant5-2

Nazia Mojib, Amin Farhoomand, Dale T. Andersen, Asim K. Bej

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In this paper, we describe the UV and cold tolerance of a purple violet pigment (PVP)-producing Antarctic bacterium, Janthinobacterium sp. Ant5-2 (PVP+) and compared its physiological adaptations with a pigmentless mutant strain (PVP-). A spontaneous deletion of vioA that codes for tryptophan monooxygenase, the first gene involved in the biosynthesis of PVP was found in PVP- strain. The PVP- culture exhibited significantly reduced survival during exponential and stationary growth phase following exposure to UVB (320 nm) and UVC (254 nm) (dose range: 0-300 J/m2) when compared to wild-type (PVP+) cultures. In addition, upon biochemical inhibition of pigment synthesis by 2(5H)-furanone, wild-type PVP+ cultures exhibited approximately 50-fold growth reduction at a higher dose (300 J/m2) of UV. Increased resistance to UV was observed upon inducing starvation state in both PVP+ and PVP- cultures. There was 80 % (SD = ±8) reduction in extrapolymeric substance (EPS) production in the PVP- cultures along with a compromised survival to freeze-thaw cycles when compared to the PVP+ cultures. Perhaps synthesis of PVP and EPS are among the key adaptive features that define the survival of this bacterium in Antarctic extreme conditions, especially during austral summer months. © 2013 Springer Japan.
Original languageEnglish (US)
Pages (from-to)367-378
Number of pages12
JournalExtremophiles
Volume17
Issue number3
DOIs
StatePublished - Mar 20 2013

ASJC Scopus subject areas

  • Molecular Medicine
  • Microbiology

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