Use of chemostat data for modelling extracellular-inulinase production by Kluyveromyces marxianus in a high-cell-density fed-batch process

Marco C.M. Hensing, Johannes Vrouwenvelder, Chris Hellinga, Johannes P. Van Dijken, Jack T. Pronk*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Production of extracellular inulinase by low-cell-density (2 kg dry weight·m-3) sucrose-limited chemostat cultures of Kluyveromyces marxianus obeyed saturated kinetics at dilution rates ranging from 0.02 to 0.5 h-1. A non-structured Monod-type equation, describing the relation between specific growth rate and specific extracellular-inulinase production rate, was used to fit experimental data. THis equation was subsequently incorporated in a model for the production of biomass and extracellular inulinase in a high-cell-density (> 100 kg dry weight·m-3) fed-batchculture of K. marxianus grown on sucrose. The model adequately described biomass production in the fed-batch culture. However, the production of extracellular inulinase in the fed-batch process was slightly higher than predicted by the model. This observation may be related to differences in growth conditions between in the chemostat and fed-batch cultures.

Original languageEnglish (US)
Pages (from-to)54-58
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number1
DOIs
StatePublished - Jan 1 1995

Keywords

  • Kluyveromyces marxianus
  • chemostat
  • extracellular inulinase
  • fed-batch process
  • modelling

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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