Uncoupling of Sister Replisomes during Eukaryotic DNA Replication

Hasan Yardimci, Anna B. Loveland, Satoshi Habuchi, Antoine M. Van Oijen*, Johannes C. Walter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that they travel away from one another and thus function independently. Alternatively, sister replisomes may form a stationary, functional unit that draws parental DNA toward itself. If this "double replisome" model is correct, a constrained DNA molecule should not undergo replication. To test this prediction, lambda DNA was stretched and immobilized at both ends within a microfluidic flow cell. Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication by a single pair of diverging replisomes. The data show that there is no obligatory coupling between sister replisomes and, together with other studies, imply that genome duplication involves autonomously functioning replisomes.

Original languageEnglish (US)
Pages (from-to)834-840
Number of pages7
JournalMolecular Cell
Volume40
Issue number5
DOIs
StatePublished - Dec 10 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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