Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy

Elvira Parrotta, Maria Teresa De Angelis, Stefania Scalise, Patrizio Candeloro, Gianluca Santamaria, Mariagrazia Paonessa, Maria Laura Coluccio, Gerardo Perozziello, Stefania De Vitis, Antonella Sgura, Elisa Coluzzi, Vincenzo Mollace, Enzo M. Di Fabrizio, Giovanni Cuda

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Background: Human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells, hold enormous promise for many biomedical applications, such as regenerative medicine, drug testing, and disease modeling. Although induced pluripotent stem cells resemble embryonic stem cells both morphologically and functionally, the extent to which these cell lines are truly equivalent, from a molecular point of view, remains controversial. Methods: Principal component analysis and K-means cluster analysis of collected Raman spectroscopy data were used for a comparative study of the biochemical fingerprint of human induced pluripotent stem cells and human embryonic stem cells. The Raman spectra analysis results were further validated by conventional biological assays. Results: Raman spectra analysis revealed that the major difference between human embryonic stem cells and induced pluripotent stem cells is due to the nucleic acid content, as shown by the strong positive peaks at 785, 1098, 1334, 1371, 1484, and 1575 cm–1, which is enriched in human induced pluripotent stem cells. Conclusions: Here, we report a nonbiological approach to discriminate human induced pluripotent stem cells from their native embryonic stem cell counterparts.
Original languageEnglish (US)
JournalStem Cell Research & Therapy
Volume8
Issue number1
DOIs
StatePublished - Nov 28 2017

Bibliographical note

KAUST Repository Item: Exported on 2021-02-10
Acknowledgements: This research was supported in part by a grant from the Programma Operativo Nazionale 2007/2013: iCARE (Infrastruttura Calabrese per la Medicina Rigenerativa: Generazione di Biobanche per la Preservazione di Cellule Staminali Umane e di Tessuto Osseo per Uso Clinico e Design e Sviluppo di Bioscaffold Innovativi) (PON03PE_00009_2) and by the Programma Operativo Nazionale 2007/2013 PON: “Exchanger: Share Your Science” (PON04a3_00433).

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