Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain

Alka Saxena; Akiko Wagatsuma; Yukihiko Noro; Takenobu Kuji; Atsuko Asaka-Oba; Akira Watahiki; Cecile Gurnot; Michela Fagiolini; Takao K. Hensch; Piero Carninci

doi:10.2144/0000113878

Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain

Alka Saxena, Akiko Wagatsuma, Yukihiko Noro, Takenobu Kuji, Atsuko Asaka-Oba, Akira Watahiki, Cecile Gurnot, Michela Fagiolini, Takao K. Hensch, Piero Carninci^*

^*Corresponding author for this work

Computational Bioscience Research Center

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.

Original language	English (US)
Pages (from-to)	381-385
Number of pages	5
Journal	BioTechniques
Volume	52
Issue number	6
DOIs	https://doi.org/10.2144/0000113878
State	Published - Jun 1 2012

Keywords

FACS
Nano CAGE
Parvalbumin
Pyramidal
RNA seq

ASJC Scopus subject areas

Biotechnology
Biochemistry, Genetics and Molecular Biology(all)

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10.2144/0000113878

Cite this

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title = "Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain",

abstract = "Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.",

keywords = "FACS, Nano CAGE, Parvalbumin, Pyramidal, RNA seq",

author = "Alka Saxena and Akiko Wagatsuma and Yukihiko Noro and Takenobu Kuji and Atsuko Asaka-Oba and Akira Watahiki and Cecile Gurnot and Michela Fagiolini and Hensch, {Takao K.} and Piero Carninci",

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Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain. / Saxena, Alka; Wagatsuma, Akiko; Noro, Yukihiko; Kuji, Takenobu; Asaka-Oba, Atsuko; Watahiki, Akira; Gurnot, Cecile; Fagiolini, Michela; Hensch, Takao K.; Carninci, Piero.

In: BioTechniques, Vol. 52, No. 6, 01.06.2012, p. 381-385.

Research output: Contribution to journal › Article › peer-review

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T1 - Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain

AU - Saxena, Alka

AU - Wagatsuma, Akiko

AU - Noro, Yukihiko

AU - Kuji, Takenobu

AU - Asaka-Oba, Atsuko

AU - Watahiki, Akira

AU - Gurnot, Cecile

AU - Fagiolini, Michela

AU - Hensch, Takao K.

AU - Carninci, Piero

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.

AB - Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.

KW - FACS

KW - Nano CAGE

KW - Parvalbumin

KW - Pyramidal

KW - RNA seq

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Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain

Abstract

Keywords

ASJC Scopus subject areas

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