Transposon-assisted genetic engineering with mos1- mediated single-copy insertion (MosSCI)

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

12 Scopus citations

Abstract

Transgenesis in model organisms is necessary to determine the function, expression, and subcellular localization of gene products. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extrachromosomal arrays but transgenes in arrays are mosaic, over-expressed in some tissues and silenced in the germline. Here, a method to insert a transgene into a specific genomic location called Mos1- mediated single-copy insertion (MosSCI) is described. Single-copy insertion allows transgene expression at levels that approximate endogenous gene expression as well as expression in the germline.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages49-58
Number of pages10
DOIs
StatePublished - Jan 1 2015

Publication series

NameMethods in Molecular Biology
Volume1327
ISSN (Print)1064-3745

Keywords

  • Endogenous levels of gene expression
  • Germline expression
  • Mos1 single-copy insertion (MosSCI)
  • Mos1 transposon
  • Transgenesis
  • Universal insertion sites

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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