Transcriptional interferences in cis natural antisense transcripts of humans and mice

Naoki Osato, Yoshiyuki Suzuki, Kazuho Ikeo, Takashi Gojobori*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

For a significant fraction of mRNAs, their expression is regulated by other RNAs, including cis natural antisense transcripts (cis -NATs) that are complementary mRNAs transcribed from opposite strands of DNA at the same genomic locus. The regulatory mechanism of mRNA expression by cis -NATs is unknown, although a few possible explanations have been proposed. To understand this regulatory mechanism, we conducted a large-scale analysis of the currently available data and examined how the overlapping arrangements of cis -NATs affect their expression level. Here, we show that for both human and mouse the expression level of cis -NATs decreases as the length of the overlapping region increases. In particular, the proportions of the highly expressed cis -NATs in all cis -NATs examined were ∼36 and 47% for human andmouse, respectively, when the overlapping region was <200 bp. However, both proportions decreased to virtually zero when the overlapping regions were >2000 bp in length. Moreover, the distribution of the expression level of cis -NATs changes according to different types of the overlapping pattern of cis -NATs in the genome. These results are consistent with the transcriptional collision model for the regulatory mechanism of gene expression by cis -NATs.

Original languageEnglish (US)
Pages (from-to)1299-1306
Number of pages8
JournalGenetics
Volume176
Issue number2
DOIs
StatePublished - Jun 2007
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint

Dive into the research topics of 'Transcriptional interferences in cis natural antisense transcripts of humans and mice'. Together they form a unique fingerprint.

Cite this