Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells

FANTOM Consortium

Research output: Contribution to journalArticlepeer-review

299 Scopus citations

Abstract

Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets of genes has not been previously studied en masse. Exploiting the fact that active promoters and enhancers are transcribed, we simultaneously measured their activity in 19 human and 14 mouse time courses covering a wide range of cell types and biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated the earliest responses. Binding sites for key lineage transcription factors were simultaneously overrepresented in enhancers and promoters active in each cellular system. Our data support a highly generalizable model in which enhancer transcription is the earliest event in successive waves of transcriptional change during cellular differentiation or activation.

Original languageEnglish (US)
Pages (from-to)1010-1014
Number of pages5
JournalScience
Volume347
Issue number6225
DOIs
StatePublished - Feb 27 2015

ASJC Scopus subject areas

  • General

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