Towards the development of a supercritical carbon dioxide spray process to coat solid protein particles

Miao Yu*, Ahmad S. Sediq, Shiduo Zhang, M. Reza Nejadnik, Hayley A. Every, Wim Jiskoot, Geert Jan Witkamp

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The aim of this study was to develop a supercritical carbon dioxide (scCO2) spray process to coat solid protein particles with a hydrophilic polymer. The final purpose is to manufacture drug particles exhibiting controlled release behaviour in patients. Lysozyme microparticles (about 20 μm) were suspended in a vessel into which a dextran sulphate (DS) solution was dispersed by scCO2 via a nozzle. Upon interaction with the droplets, DS was deposited onto or mixed with suspended lysozyme particles. Particles of about 100 μm were obtained. The zeta-potential analysis and elemental analysis indicated that the top layer of the particles consisted of both lysozyme and DS. Some of the produced particulate materials showed retarded lysozyme release when exposed to water or phosphate buffered saline, holding promise for future production of controlled drug delivery systems for therapeutic proteins.

Original languageEnglish (US)
Pages (from-to)49-59
Number of pages11
JournalJournal of Supercritical Fluids
Volume141
DOIs
StatePublished - Nov 2018

Keywords

  • Dextran sulphate
  • Drug delivery systems
  • Lysozyme
  • Proteins
  • Supercritical carbon dioxide

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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