Towards a high throughput droplet-based agglutination assay

Rimantas Kodzius, David Castro, Ian G. Foulds

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    This work demonstrates the detection method for a high throughput droplet based agglutination assay system. Using simple hydrodynamic forces to mix and aggregate functionalized microbeads we avoid the need to use magnetic assistance or mixing structures. The concentration of our target molecules was estimated by agglutination strength, obtained through optical image analysis. Agglutination in droplets was performed with flow rates of 150 µl/min and occurred in under a minute, with potential to perform high-throughput measurements. The lowest target concentration detected in droplet microfluidics was 0.17 nM, which is three orders of magnitude more sensitive than a conventional card based agglutination assay.
    Original languageEnglish (US)
    Title of host publicationThe 7th International Conference on Microtechnologies in Medicine and Biology MMB
    PublisherThe 7th International Conference on Microtechnologies in Medicine and Biology MMB
    StatePublished - Oct 22 2013

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