Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Tristan Colomb*, Jonas Kühn, Florian Charrière, Christian Depeursinge, Pierre Marquet, Nicolas Aspert

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

244 Scopus citations

Abstract

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram. We demonstrate that in particular cases in which the studied specimen does not have abrupt edges, the specimen's hologram itself can be used as reference hologram. We show that image distortion and phase aberrations introduced by a lens ball used as microscope objective are completely suppressed with our method. Finally the concept of self-conjugated reference hologram is applied on a biological sample (Trypanosoma Brucei) to maintain a spatial phase noise level under 3 degrees.

Original languageEnglish (US)
Pages (from-to)4300-4306
Number of pages7
JournalOptics Express
Volume14
Issue number10
DOIs
StatePublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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