Topical Effect of a Medically Prescribed Pediatric Antibiotic on Dental Biofilm: A Cross-Over, In Situ Study

Viviane Santos da Silva Pierro, Dennis de Carvalho Ferreira, Hugo Emiliano de Jesus, Alexandre Soares Rosado, Ronir Raggio Luiz, Kátia Regina Netto dos Santos, Lucianne Cople Maia

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Objective: This study aimed to investigate the possible topical effect of a broad-spectrum antibiotic on dental biofilm formed in situ in the absence or presence of sucrose. Methods: A crossover study was conducted in three phases of 14 days each, during which 11 volunteers wore palatal devices containing 6 enamel blocks covered with meshes to allow biofilm formation. Dental blocks were extraorally submitted to a 20% sucrose solution at three different frequencies of exposure (0, 3 and 8 times/day), and to a suspension of amoxicillin/clavulanate potassium (A/CP) or a placebo (P) suspension at an 8-hour time interval application regimen. On the 14th day of each phase, biofilms were collected for microbiological (conventional culture) and molecular (Denaturing Gradient Gel Electrophoresis - DGGE) analyses. Results: In the absence of sucrose exposure (SE) and at the 3-time daily frequency, dental biofilms treated with A/CP showed lower total biofilm weight and lower counts of total microbiota than the ones treated with P (p>0.05). A/CP presented higher counts of Candida spp. when compared with P in the presence of SE, especially at the 8-time daily frequency (p0.05), regardless of SE. However, DGGE profiles demonstrated large interindividual variability. Conclusion: Both conventional culture and DGGE have demonstrated some differences on total microbiota of dental biofilms when exposed to the A/CP or P suspensions, mainly in the absence of sucrose, which suggests a possible topical effect of the sugar-free A/CP suspension on dental biofilm. © 2013 Pierro et al.
Original languageEnglish (US)
JournalPLoS ONE
Volume8
Issue number1
DOIs
StatePublished - Jan 31 2013
Externally publishedYes

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