Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy

Paolo Galll, Giovanni Strona, Anna Maria Villa, Francesca Benzoni, Stefani Fabrizio, Silvia Maria Doglia, Delane C. Kritsky

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A nondestructive protocol for preparing specimens of Monogenoidea for both α-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy. © American Society of Parasitologists 2006.
Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalJournal of Parasitology
Volume92
Issue number2
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

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