Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape

Kholod Alamoudi, Patricia Martins, Jonas G. Croissant, Sachin Patil, Haneen Omar, Niveen M. Khashab

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.
Original languageEnglish (US)
Pages (from-to)1421-1433
Number of pages13
JournalNanomedicine
Volume12
Issue number12
DOIs
StatePublished - May 19 2017

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