The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum

Wendelin Schnippenkoetter, Clive Lo, Guoquan Liu, Katherine Dibley, Wai Lung Chan, Jodie White, Ricky Milne, Alexander Zwart, Eunjung Kwong, Beat Keller, Ian Godwin, Simon G. Krattinger, Evans Lagudah*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The ability of the wheat Lr34 multipathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen Puccinia purpurea and the hemibiotroph Colletotrichum sublineolum, respectively, occurred in transgenic plants expressing the Lr34res ABC transporter. Transgenic sorghum lines that highly expressed the wheat Lr34res gene exhibited immunity to sorghum rust compared to the low-expressing single copy Lr34res genotype that conferred partial resistance. Pathogen-induced pigmentation mediated by flavonoid phytoalexins was evident on transgenic sorghum leaves following P. purpurea infection within 24–72 h, which paralleled Lr34res gene expression. Elevated expression of flavone synthase II, flavanone 4-reductase and dihydroflavonol reductase genes which control the biosynthesis of flavonoid phytoalexins characterized the highly expressing Lr34res transgenic lines 24-h post-inoculation with P. purpurea. Metabolite analysis of mesocotyls infected with C. sublineolum showed increased levels of 3-deoxyanthocyanidin metabolites were associated with Lr34res expression, concomitant with reduced symptoms of anthracnose.

Original languageEnglish (US)
Pages (from-to)1387-1396
Number of pages10
JournalPlant Biotechnology Journal
Volume15
Issue number11
DOIs
StatePublished - Nov 2017

Keywords

  • Lr34
  • anthracnose
  • flavonoid phytoalexin
  • multiple disease resistance
  • rust

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Fingerprint

Dive into the research topics of 'The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum'. Together they form a unique fingerprint.

Cite this