The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase

Severine Hurni, Daniela Scheuermann, Simon Krattinger, Bettina Kessel, Thomas Wicker, Gerhard Herren, Mirjam N. Fitze, James Breen, Thomas Presterl, Milena Ouzunova, Beat Keller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

134 Scopus citations

Abstract

Northern corn leaf blight (NCLB) caused by the hemibiotrophic fungus Exserohilum turcicum is an important foliar disease of maize that is mainly controlled by growing resistant maize cultivars. The Htn1 locus confers quantitative and partial NCLB resistance by delaying the onset of lesion formation. Htn1 represents an important source of genetic resistance that was originally introduced from a Mexican landrace into modern maize breeding lines in the 1970s. Using a high-resolution map-based cloning approach, we delimited Htn1 to a 131.7-kb physical interval on chromosome 8 that contained three candidate genes encoding two wall-associated receptor-like kinases (ZmWAK-RLK1 and ZmWAK-RLK2) and one wall-associated receptor-like protein (ZmWAKRLP1). TILLING (targeting induced local lesions in genomes) mutants in ZmWAK-RLK1 were more susceptible to NCLB than wild-type plants, both in greenhouse experiments and in the field. ZmWAK-RLK1 contains a nonarginine-aspartate (non-RD) kinase domain, typically found in plant innate immune receptors. Sequence comparison showed that the extracellular domain of ZmWAK-RLK1 is highly diverse between different maize genotypes. Furthermore, an alternative splice variant resulting in a truncated protein was present at higher frequency in the susceptible parents of the mapping populations compared with in the resistant parents. Hence, the quantitative Htn1 disease resistance in maize is encoded by an unusual innate immune receptorwith an extracellular wall-associated kinase domain. These results further highlight the importance of this protein family in resistance to adapted pathogens.

Original languageEnglish (US)
Pages (from-to)8780-8785
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number28
DOIs
StatePublished - Jul 14 2015

Keywords

  • Exserohilum turcicum
  • Maize
  • Pattern recognition receptor
  • Quantitative disease resistance
  • Wall-associated receptor-like kinase

ASJC Scopus subject areas

  • General

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