The isolated retina of mammals: A useful preparation for enzymatic-(adenylyl cyclase) and/or binding studies of dopamine receptors

M. Schorderet*, Pierre Magistretti

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Rabbit and bovine retinae were used for investigations in vitro related to dopamine receptors. Dose-dependent stimulations of cyclic AMP formation induced by dopamine (or dopamine-like drugs including ADTN and some ergot alkaloid derivatives), which can be blocked by dopamine antagonists (neuroleptics), lithium or d-LSD, were found either in intact retinae or in homogenates. The neuroleptic sulpiride was the only exception since it was devoid of inhibitory effects. Binding studies of retinal dopamine receptors were also achieved with homogenates. By using 3H-spiroperidol as a ligand, saturability (in the amolar range), stereospecificity (by using butaclamol- or thioxanthene-isomers), tissue linearity, and pH- or temperature-dependence were all demonstrated. Furthermore, displacement curves of 3H-spiroperidol stereospecifically bound generated by various dopamineand/or catecholamine-related drugs seem to indicate that dopamine receptors are the only monoamine receptors in this tissue and that a coupling possibly exists between binding- and catalytic-sites. These results suggest that by studying both dopamine-sensitive adenylyl cyclase and drug-induced displacement of 3H-spiroperidol binding the mammalian retina is appropriate to selectively characterize the type of action of various drugs with CNS dopamine receptors.

Original languageEnglish (US)
Pages (from-to)337-353
Number of pages17
JournalNeurochemistry International
Volume1
Issue numberC
DOIs
StatePublished - Jan 1 1980

Keywords

  • Retina dopamine receptors
  • adenylyl cyclase
  • binding studies
  • ergot alkaloids
  • neuroleptics

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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