The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells

Jian Li*, Brigitte Happ, Christian Schetter, Cornelia Oellig, Charlotte Hauser, Kazumichi Kuroda, Dagmar Knebel-Mörsdorf, Hans Dieter Klenk, Walter Doerfler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

This report presents a synopsis of recently published work in our laboratory on the molecular biology of the insect baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The following studies have been summarized. 1. (1) On the mode of transcription of the AcNPV genome in insect cells. 2. (2) Translation of proteins encoded in the 8.12 to 85.0 map unit segment of AcNPV. 3. (3) Inserts of insect cell DNA in the AcNPV genome. 4. (4) Expression of influenza (fowl plague) virus haemagglutinin in Spodoptera frugiperda insect cells, and successful immunization of chickens. 5. (5) Synthesis of the influenza virus haemagglutinin in insect larvae by recombinant AcNPV. This insect virus system will continue to serve as a model for research on the molecular biology of insects. Moreover, the baculovirus system has been recognized as a very efficient and safe eukaryotic expression vector.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalVeterinary Microbiology
Volume23
Issue number1-4
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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