The effect of exogenous nitrate on LCO signalling, cytokinin accumulation, and nodule initiation in Medicago truncatula

Kerstin Gühl, Rens Holmer, Ting Ting Xiao, Defeng Shen, Titis A.K. Wardhani, René Geurts, Arjan van Zeijl, Wouter Kohlen

Research output: Contribution to journalArticlepeer-review

Abstract

Nitrogen fixation by rhizobia is a highly energy-demanding process. Therefore, nodule initiation in legumes is tightly regulated. Environmental nitrate is a potent inhibitor of nodulation. However, the precise mechanism by which this agent (co)regulates the inhibition of nodulation is not fully understood. Here, we demonstrate that in Medicago truncatula the lipo-chitooligosaccharideinduced accumulation of cytokinins is reduced in response to the application of exogenous nitrate. Under permissive nitrate conditions, perception of rhizobia-secreted signalling molecules leads to an increase in the level of four cytokinins (i.e., iP, iPR, tZ, and tZR). However, under high-nitrate conditions, this increase in cytokinins is reduced. The ethylene-insensitive mutant Mtein2/sickle, as well as wild-type plants grown in the presence of the ethylene biosynthesis inhibitor 2-aminoethoxyvinyl glycine (AVG), is resistant to the inhibition of nodulation by nitrate. This demonstrates that ethylene biosynthesis and perception are required to inhibit nodule organogenesis under high-nitrate conditions.
Original languageEnglish (US)
Pages (from-to)988
JournalGenes
Volume12
Issue number7
DOIs
StatePublished - Jun 28 2021

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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