The cdc2Ms kinase is differently regulated in the cytoplasm and in the nucleus

László Bögre*, Karin Zwerger, Irute Meskiene, Pavla Binarova, Vilmos Csizmadia, Christian Planck, Ernst Wagner, Heribert Hirt, Erwin Heberle-Bors

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

To study a cyclin-dependent kinase (CDK) from alfalfa (Medicago sativa L.), an antibody was raised against the C-terminal 16 amino acids of the protein cdc2aMs. The cdc2Ms protein was immunopurified with this antibody and its histone kinase activity was measured. The cdc2Ms kinase is activated at the G1/S transition when phosphate-starved cells from the G0 phase re-enter the cell cycle and remain active as cells transit the S, G2, and M phases, indicating that the same CDK regulates all of these phases in alfalfa. In contrast, when cdc2Ms kinase was purified by binding to p13(suc1), it was active only in the G2 and M phases. In immunoblots the C-terminal antibody detected an equal amount of the cdc2Ms protein in the cytoplasm and in the nucleus. By indirect immunofluorescence, however, the cytoplasmic form of cdc2Ms could not be found in the S phase of the cells, indicating that the epitope for the cdc2 antibody is not accessible. Binding of putative inhibitor proteins to cdc2 was shown by inactivation of purified plant CDK when cell extracts were added. Furthermore, purified CDK inhibitors, such as the mouse p27(kip1) and the yeast p40(sic1), blocked the purified plant CDK activity.

Original languageEnglish (US)
Pages (from-to)841-852
Number of pages12
JournalPlant physiology
Volume113
Issue number3
DOIs
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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