The BAF60 subunit of the SWI/SNF chromatin-remodeling complex directly controls the formation of a gene loop at FLOWERING LOCUS C in Arabidopsis

Teddy Jégu, David Latrasse, Marianne Delarue, Heribert Hirt, Séverine Domenichini, Federico Ariel, Martin Crespi, Catherine Bergounioux, Cécile Raynaud, Moussa Benhamed*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

SWI/SNF complexes mediate ATP-dependent chromatin remodeling to regulate gene expression. Many components of these complexes are evolutionarily conserved, and several subunits of Arabidopsis thaliana SWI/SNF complexes are involved in the control of flowering, a process that depends on the floral repressor FLOWERING LOCUS C (FLC). BAF60 is a SWI/SNF subunit, and in this work, we show that BAF60, via a direct targeting of the floral repressor FLC, induces a change at the high-order chromatin level and represses the photoperiod flowering pathway in Arabidopsis. BAF60 accumulates in the nucleus and controls the formation of the FLC gene loop by modulation of histone density, composition, and posttranslational modification. Physiological analysis of BAF60 RNA interference mutant lines allowed us to propose that this chromatin-remodeling protein creates a repressive chromatin configuration at the FLC locus.

Original languageEnglish (US)
Pages (from-to)538-551
Number of pages14
JournalPlant Cell
Volume26
Issue number2
DOIs
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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