The association of heavy and light chain variable domains in antibodies: implications for antigen specificity.

Anna Chailyan, Paolo Marcatili, Anna Tramontano

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The antigen-binding site of immunoglobulins is formed by six regions, three from the light and three from the heavy chain variable domains, which, on association of the two chains, form the conventional antigen-binding site of the antibody. The mode of interaction between the heavy and light chain variable domains affects the relative position of the antigen-binding loops and therefore has an effect on the overall conformation of the binding site. In this article, we analyze the structure of the interface between the heavy and light chain variable domains and show that there are essentially two different modes for their interaction that can be identified by the presence of key amino acids in specific positions of the antibody sequences. We also show that the different packing modes are related to the type of recognized antigen.
Original languageEnglish (US)
Pages (from-to)2858-2866
Number of pages9
JournalFEBS Journal
Volume278
Issue number16
DOIs
StatePublished - Jun 28 2011
Externally publishedYes

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