Tbx genes and limb identity in chick embryo development

Alison Isaac*, Concepción Rodriguez-Esteban, Aimee Ryan, Muriel Altabef, Tohru Tsukui, Ketan Patel, Cheryll Tickle, Juan Carlos Izpisúa-Belmonte

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

134 Scopus citations

Abstract

Tbx-2, Tbx-3, Tbx-4 and Tbx-5 chick genes have been isolated and, like the mouse homologues, are expressed in the limb regions. Tbx-2 and Tbx-3 are expressed in anterior and posterior domains in wings and legs, as well as throughout the flank. Of particular interest, however, are Tbx-5, which is expressed in wing and flank but not leg, and Tbx-4, which is expressed very strongly in leg but not wing. Grafts of leg tissue to wing and wing tissue to leg give rise to toe-like or wing-like digits in wing and leg respectively. Expression of Tbx-4 is stable when leg tissue is grafted to wing, and Tbx-5 expression is stable when wing tissue is grafted to leg. Induction of either extra wings or legs from the flank by applying FGF-2 in different positions alters the expression of Tbx-4 and Tbx-5 in such a way that suggests that the amount of Tbx-4 that is expressed in the limb determines the type that will form. The ectopic limb always displays a limb-like Tbx-3 expression. Thus Tbx-4 and Tbx-5 are strong candidates for encoding 'wingness' and 'legness'.

Original languageEnglish (US)
Pages (from-to)1867-1875
Number of pages9
JournalDevelopment
Volume125
Issue number10
StatePublished - 1998
Externally publishedYes

Keywords

  • Chick embryos
  • FGF-2
  • Limb development
  • Limb mesoderm
  • Tbx genes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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