TY - JOUR
T1 - Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein
AU - Mahfouz, Magdy M.
AU - Li, Lixin
AU - Piatek, Marek J.
AU - Fang, Xiaoyun
AU - Mansour, Hicham
AU - Bangarusamy, Dhinoth Kumar
AU - Zhu, Jian-Kang
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2011/12/14
Y1 - 2011/12/14
N2 - Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29A
AB - Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29A
UR - http://hdl.handle.net/10754/325247
UR - http://link.springer.com/10.1007/s11103-011-9866-x
UR - http://www.scopus.com/inward/record.url?scp=84855882014&partnerID=8YFLogxK
U2 - 10.1007/s11103-011-9866-x
DO - 10.1007/s11103-011-9866-x
M3 - Article
C2 - 22167390
VL - 78
SP - 311
EP - 321
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 3
ER -