Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein

Magdy M. Mahfouz, Lixin Li, Marek J. Piatek, Xiaoyun Fang, Hicham Mansour, Dhinoth Kumar Bangarusamy, Jian-Kang Zhu

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3. SRDX protein efficiently repressed the transcription of the RD29A
Original languageEnglish (US)
Pages (from-to)311-321
Number of pages11
JournalPlant Molecular Biology
Volume78
Issue number3
DOIs
StatePublished - Dec 14 2011

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

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