Targeted misexpression of Hox-4.6 in the avian limb bud causes apparent homeotic transformations

Bruce A. Morgan*, Juan Carlos Izpisua Belmonte, Denis Duboule, Clifford J. Tabin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

258 Scopus citations

Abstract

IN the limb bud the 5' members of the Hox-4 gene cluster are expressed in a nested set of overlapping domains which are progressively restricted in the posterior and distal directions1. These domains arise early in limb bud development and come to approximate the primordia of the major structural elements of the limb along the anterior/posterior axis2 (Fig. 1). This pattern, and the fact that surgical manipulations which lead to mirror image duplications along the anterior/posterior axis give rise to mirror image duplications of the domains of expression of these genes, have led to the proposal that these transcription factors specify positional identity along the anterior/posterior axis3,4. Here we test this hypothesis directly using replication-competent retroviral vectors to expand the domain of expression of the Hox-4.6 gene anteriorly during limb development in vivo.We report that alteration of the domain of expression of the Hox-4.6 gene in the developing limb leads to reproducible pattern alterations consistent with a posterior homeotic transformation.

Original languageEnglish (US)
Pages (from-to)236-239
Number of pages4
JournalNature
Volume358
Issue number6383
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • General

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