Targeted gene deletions in C. elegans using transposon excision

Christian Froekjaer Jensen, M. Wayne Davis, Gunther Hollopeter, Jon Taylor, Todd W. Harris, Paola Nix, Rachel Lofgren, Michael Prestgard-Duke, Michael Bastiani, Donald G. Moerman, Erik M. Jorgensen

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

Original languageEnglish (US)
Pages (from-to)451-453
Number of pages3
JournalNature Methods
Volume7
Issue number6
DOIs
StatePublished - Jun 1 2010

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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