TALE nucleases and next generation GM crops.

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Site-specific and adaptable DNA binding domains are essential modules to develop genome engineering technologies for crop improvement. Transcription activator-like effectors (TALEs) proteins are used to provide a highly specific and adaptable DNA binding modules. TALE chimeric nucleases (TALENs) were used to generate site-specific double strand breaks (DSBs) in vitro and in yeast, Caenorhabditis elegans, mammalian and plant cells. The genomic DSBs can be generated at predefined and user-selected loci and repaired by either the non-homologous end joining (NHEJ) or homology dependent repair (HDR). Thus, TALENs can be used to achieve site-specific gene addition, stacking, deletion or inactivation. TALE-based genome engineering tools should be powerful to develop new agricultural biotechnology approaches for crop improvement. Here, we discuss the recent research and the potential applications of TALENs to accelerate the generation of genomic variants through targeted mutagenesis and to produce a non-transgenic GM crops with the desired phenotype.
Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalGM crops
Volume2
Issue number2
DOIs
StatePublished - Apr 1 2011

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint

Dive into the research topics of 'TALE nucleases and next generation GM crops.'. Together they form a unique fingerprint.

Cite this