System-wide detection of protein-small molecule complexes suggests extensive metabolite regulation in plants

Daniel Veyel, Sylwia Kierszniowska, Monika Kosmacz, Ewelina Maria Sokolowska, Aenne Michaelis, Marcin Luzarowski, Jagoda Szlachetko, Lothar Willmitzer, Aleksandra Skirycz

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Protein small molecule interactions are at the core of cell regulation controlling metabolism and development. We reasoned that due to the lack of system wide approaches only a minority of those regulatory molecules are known. In order to see whether or not this assumption is true we developed an effective approach for the identification of small molecules having potential regulatory role that obviates the need of protein or small molecule baits. At the core of this approach is a simple biochemical co-fractionation taking advantage of size differences between proteins and small molecules. Metabolomics based analysis of small molecules co-fractionating with proteins identified a multitude of small molecules in Arabidopsis suggesting the existence of numerous, small molecules/metabolites bound to proteins representing potential regulatory molecules. The approach presented here uses Arabidopsis cell cultures, but is generic and hence applicable to all biological systems.
Original languageEnglish (US)
JournalScientific Reports
Volume7
DOIs
StatePublished - Feb 13 2017
Externally publishedYes

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