Abstract
Site-directed conjugation of metal centers to proteins is fundamental for biological and bioinorganic applications of transition metals. However, methods for the site-selective introduction of metal centers remain scarce. Herein, we present broadly applicable synthetic strategies for the conjugation of bioactive molecules with a range of organometallic complexes. Following three different synthetic strategies, we were able to synthesize a small library of metal conjugated protein markers featuring different types of protein reactive sites (epoxides, phenylphosphonates, fluorosulfonates and fluorophosphonate groups) as well as different late transition metals (iron, ruthenium, rhodium, palladium and platinum). The products were isolated in moderate to excellent yields and high purity. Furthermore, X-ray diffraction of the metalated protein markers corroborates structural integrity of the metal complex and the protein reactive site. © 2013 Elsevier B.V. All rights reserved.
Original language | English (US) |
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Pages (from-to) | 82-91 |
Number of pages | 10 |
Journal | Journal of Organometallic Chemistry |
Volume | 744 |
DOIs | |
State | Published - Nov 2013 |
ASJC Scopus subject areas
- Materials Chemistry
- Biochemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Inorganic Chemistry
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Datasets
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CCDC 929468: Experimental Crystal Structure Determination : ((eta^5^-1-(2-((4-(fluorosulfonyl)benzoyl)amino)ethyl)-2,3,4,5-tetramethylcyclopentadienyl))-dichloro-triphenylphosphine-rhodium chloroform solvate
Jantke, D. (Creator), Marziale, A. N. (Creator), Reiner, T. (Creator), Kraus, F. (Creator), Herdtweck, E. (Creator), Raba, A. (Creator) & Eppinger, J. (Creator), Dryad Digital Repository, 2013
DOI: 10.5517/cc1065v7, http://hdl.handle.net/10754/624198
Dataset
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CCDC 868620: Experimental Crystal Structure Determination : (eta^5^-2,3,4,5-tetramethyl-1-{[(3-{[1-(benzylamino)-4-methyl-1-oxopentan-2-yl]carbamoyl}oxiran-2-yl)carbonyl]amino}ethyl-cyclopentadienyl)-dichloro-(triphenylphosphine)-rhodium unknown solvate
Jantke, D. (Creator), Marziale, A. N. (Creator), Reiner, T. (Creator), Kraus, F. (Creator), Herdtweck, E. (Creator), Raba, A. (Creator) & Eppinger, J. (Creator), Dryad Digital Repository, 2013
DOI: 10.5517/ccy4w0y, http://hdl.handle.net/10754/624685
Dataset