Synthetic histone code

Wolfgang Fischle*, Henning D. Mootz, Dirk Schwarzer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Chromatin is the universal template of genetic information in all eukaryotic cells. This complex of DNA and histone proteins not only packages and organizes genomes but also regulates gene expression. A multitude of posttranslational histone modifications and their combinations are thought to constitute a code for directing distinct structural and functional states of chromatin. Methods of protein chemistry, including protein semisynthesis, amber suppression technology, and cysteine bioconjugation, have enabled the generation of so-called designer chromatin containing histones in defined and homogeneous modification states. Several of these approaches have matured from proof-of-concept studies into efficient tools and technologies for studying the biochemistry of chromatin regulation and for interrogating the histone code. We summarize pioneering experiments and recent developments in this exciting field of chemical biology.

Original languageEnglish (US)
Pages (from-to)131-140
Number of pages10
JournalCurrent Opinion in Chemical Biology
Volume28
DOIs
StatePublished - Oct 1 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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