Synthesis of mosaic membranes and application for egg white protein fractionation by partitioned free-flow isoelectric focusing (FFIEF)

Jiu Hua Cheng, Tai-Shung Chung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In this article, multi-component protein fractionation was studied by mosaic membrane partitioned free-flow isoelectric focusing (FFIEF). The mosaic membranes were molecularly designed through a blending of aminated poly(2,6-dimethyl-1,4-phenylene oxide) (APPO) and sulfonated polysulfone (SPSf) with polysulfone (PSf). All cast membranes were prepared from the same dope but phase inverted in different coagulants to produce different morphologies. Membrane characterizations through field emission scanning electron microscopy (FESEM) imaging, streaming potential, pore size distribution and PWP (pure water permeation) demonstrated that the self-prepared membranes possessed a variety of membrane structures and charge characteristics. These mosaic membranes were applied in FFIEF as the selective barriers. Experimental results show that a blend of APPO and SPSf with PSf is an effective route to produce highly charged mosaic membranes, which can be used in the separation of five pure protein components from chicken egg white using the mosaic-membrane partitioned FFIEF.

Original languageEnglish (US)
Pages (from-to)94-102
Number of pages9
JournalJournal of Membrane Science
Volume353
Issue number1-2
DOIs
StatePublished - Mar 16 2010

Keywords

  • Electrophoresis
  • Free-flow isoelectric focusing (FFIEF)
  • Ion-exchange membranes
  • Multi-compartment electrophoresis (MCE)
  • Protein separation

ASJC Scopus subject areas

  • Biochemistry
  • Materials Science(all)
  • Physical and Theoretical Chemistry
  • Filtration and Separation

Fingerprint Dive into the research topics of 'Synthesis of mosaic membranes and application for egg white protein fractionation by partitioned free-flow isoelectric focusing (FFIEF)'. Together they form a unique fingerprint.

Cite this