TY - JOUR
T1 - Structural variations in wheat HKT1;5 underpin differences in Na+ transport capacity
AU - Xu, Bo
AU - Waters, Shane
AU - Byrt, Caitlin S.
AU - Plett, Darren
AU - Tyerman, Stephen D.
AU - Tester, Mark A.
AU - Munns, Rana
AU - Hrmova, Maria
AU - Gilliham, Mathew
N1 - KAUST Repository Item: Exported on 2021-11-30
Acknowledgements: This work was supported by the Grains Research and Development Corporation (UA00145, M.G.), the University of Adelaide Australian Postgraduate Award and the CJ Everald postgraduate scholarship (S.W.), and the Australian Research Council through the following schemes: Discovery (DP120100900, M.H.), Centre of Excellence (CE140100008, M.G., R.M., S.D.T), Future Fellowship (FT130100709, M.G.), and DECRA (DE150100837, C.S.B.).
PY - 2017/11/27
Y1 - 2017/11/27
N2 - An important trait associated with the salt tolerance of wheat is the exclusion of sodium ions (Na+) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na+-exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (Km) for the Na+ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D471/a gap and D474/G473 that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na+ exclusion in wheat that leads to an improved salinity tolerance in the field.
AB - An important trait associated with the salt tolerance of wheat is the exclusion of sodium ions (Na+) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na+-exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (Km) for the Na+ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D471/a gap and D474/G473 that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na+ exclusion in wheat that leads to an improved salinity tolerance in the field.
UR - http://hdl.handle.net/10754/626256
UR - http://link.springer.com/article/10.1007/s00018-017-2716-5
UR - http://www.scopus.com/inward/record.url?scp=85035010990&partnerID=8YFLogxK
U2 - 10.1007/s00018-017-2716-5
DO - 10.1007/s00018-017-2716-5
M3 - Article
C2 - 29177534
AN - SCOPUS:85035010990
VL - 75
SP - 1133
EP - 1144
JO - Cellular and Molecular Life Sciences
JF - Cellular and Molecular Life Sciences
SN - 1420-682X
IS - 6
ER -