Strategy for in situ detection of natural transformation-based horizontal gene transfer events

Aurora Rizzi, Alessandra Pontiroli, Lorenzo Brusetti, Sara Borin, Claudia Sorlini, Alessandro Abruzzese, Gian Attilio Sacchi, Timothy M. Vogel, Pascal Simonet, Marco Bazzicalupo, Kaare Magne Nielsen, Jean Michel Monier, Daniele Daffonchio*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.

Original languageEnglish (US)
Pages (from-to)1250-1254
Number of pages5
JournalApplied and Environmental Microbiology
Volume74
Issue number4
DOIs
StatePublished - Jan 1 2008

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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