Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes

Haïtham Sghaier, Karima Hezbri, Faten Ghodhbane-Gtari, Petar Pujic, Arnab Sen, Daniele Daffonchio, Abdellatif Boudabous, Louis S Tisa, Hans-Peter Klenk, Jean Armengaud, Philippe Normand, Maher Gtari

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The Geodermatophilaceae are unique model systems to study the ability to thrive on or within stones and their proteogenomes (referring to the whole protein arsenal encoded by the genome) could provide important insight into their adaptation mechanisms. Here we report the detailed comparative genome analysis of Blastococcus saxobsidens (Bs), Modestobacter marinus (Mm) and Geodermatophilus obscurus (Go) isolated respectively from the interior and the surface of calcarenite stones and from desert sandy soils. The genome-scale analysis of Bs, Mm and Go illustrates how adaptation to these niches can be achieved through various strategies including ‘molecular tinkering/opportunism’ as shown by the high proportion of lost, duplicated or horizontally transferred genes and ORFans. Using high-throughput discovery proteomics, the three proteomes under unstressed conditions were analyzed, highlighting the most abundant biomarkers and the main protein factors. Proteomic data corroborated previously demonstrated stone-related ecological distribution. For instance, these data showed starvation-inducible, biofilm-related and DNA-protection proteins as signatures of the microbes associated with the interior, surface and outside of stones, respectively.
Original languageEnglish (US)
Pages (from-to)21-29
Number of pages9
JournalThe ISME Journal
Volume10
Issue number1
DOIs
StatePublished - Jun 30 2015

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