Stable transfection of cDNAs targeting specific steps of glycogen metabolism supports the existence of active gluconeogenesis in mouse cultured astrocytes

Katy Bernard-Hélary, Maryvonne Ardourel, Pierre Magistretti, Tobias Hévor, Jean François Cloix*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In order to assess the participation of astrocytic gluconeogenesis in the synthesis of glycogen, mouse astrocytes were stably transfected with antisense cDNA of fructose-1,6-bisphosphatase (FBPase) and with sense and antisense cDNAs of glycogen synthase (GS). The antisenses of FBPase and GS have similar significant effect in decreasing astrocyte glycogen content by 60%, while sense GS significantly increased glycogen content by 100%. The FBPase activity was decreased by all three cDNAs used, while glycogen phosphorylase was not altered. The activity of GS was decreased by the antisense GS and increased by the sense GS. These data demonstrate that the gluconeogenesis in astrocytes is involved in the glycogenesis modulation.

Original languageEnglish (US)
Pages (from-to)379-382
Number of pages4
JournalGLIA
Volume37
Issue number4
DOIs
StatePublished - Mar 15 2002

Keywords

  • Astrocytes
  • CDNA
  • Fructose-1,6-bisphosphatase
  • Glycogen
  • Glycogen synthase
  • Transfection

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Stable transfection of cDNAs targeting specific steps of glycogen metabolism supports the existence of active gluconeogenesis in mouse cultured astrocytes'. Together they form a unique fingerprint.

Cite this