Specific hypomethylated CpGs at the IGF2 locus act as an epigenetic biomarker for familial adenomatous polyposis colorectal cancer

Audrey Miroglio, Hélne Jammes, Jrg Tost, Loc Ponger, Ivo Glynne Gut, Hafida El Abdalaoui, Jol Coste, Stanislas Chaussade, Paola B. Arimondo, Dominique Lamarque, Luisa Dandolo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Aims: The identification of specific biomarkers for colorectal cancer is of primary importance for early diagnosis. The aim of this study was to evaluate if methylation changes at the IGF2/H19 locus could be predictive for individuals at high risk for developing sporadic or hereditary colorectal cancer. Materials & methods: Quantitative methylation analysis using pyrosequencing was performed on three differentially methylated regions (DMRs): IGF2 DMR0 and DMR2 and the H19 DMR in DNA samples from sporadic colorectal cancer (n = 26), familial adenomatous polyposis (n = 35) and hereditary nonpolyposis colorectal cancer (n = 19) patients. Results: We report in this article for the first time, that in sporadic colorectal cancer tumor DNA both the IGF2 DMR0 and DMR2 are hypomethylated, while the H19 DMR retains its monoallelic methylation pattern. In lymphocyte DNA, a striking hypomethylation of nine contiguous correlated CpGs was found in the IGF2 DMR2 but only in familial adenomatous polyposis patients. Conclusion: Methylation alterations at the IGF2 locus are more extensive than previously reported and DMR2 hypomethylation in lymphocyte DNA might be a specific epigenetic biomarker for familial adenomatous polyposis patients.

Original languageEnglish (US)
Pages (from-to)365-375
Number of pages11
JournalEpigenomics
Volume2
Issue number3
DOIs
StatePublished - Jun 1 2010

Keywords

  • DNA methylation
  • colorectal cancer
  • epigenetics
  • lymphocytes
  • pyrosequencing

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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