Specific Bacillus anthracis identification by a plcR-targeted restriction site insertion-PCR (RSI-PCR) assay

Rafał Gierczyński*, Aleksandra A. Zasada, Noura Raddadi, Maya Merabishvili, Daniele Daffonchio, Waldemar Rastawicki, Marek Jagielski

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A RSI-PCR assay was developed for the detection of a Bacillus anthracis-specific nonsense mutation in the plcR gene. The assay specificity was tested using 170 Bacillus spp. strains including 47 strains of B. anthracis. The plcR RSI-PCR distinguished Bacillus cereus group strains closely related to B. anthracis from the anthrax agent. The assay was found to be a robust, simple and cost effective tool for B. anthracis identification. In contrast to previously developed real time PCR-based methods, the RSI-PCR needs basic molecular biology equipment only, and thus may be easily introduced in developing countries, where anthrax is endemic.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalFEMS Microbiology Letters
Volume272
Issue number1
DOIs
StatePublished - Jul 1 2007

Keywords

  • Anthrax
  • Bacillus anthracis
  • RSI-PCR
  • plcR

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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