Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein

Satoshi Habuchi, Mircea Cotlet, Roel Gronheid, Gunter Dirix, Jan Michiels, Jos Vanderleyden, Frans C. De Schryver, Johan Hofkens*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

139 Citations (SciVal)

Abstract

In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.

Original languageEnglish (US)
Pages (from-to)8446-8447
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number28
DOIs
StatePublished - Jul 16 2003

ASJC Scopus subject areas

  • Catalysis
  • Biochemistry
  • Chemistry(all)
  • Colloid and Surface Chemistry

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