Single-molecule imaging with an inexpensive UV-LED light source

Akifumi Hattori, Satoshi Habuchi, Martin Vacha*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The powerful tool of single-molecule detection and spectroscopy has so far been confined mainly to the visible spectral region. Here, we report the use of a low-cost high-power UV light emitting diode (LED) emitting at 365 nm as a light source for single-molecule detection in epi-fluorescence microscopy. The LED provides an affordable alternative to UV laser systems. The performance of the light source is demonstrated by recording fluorescence images and time-dependent fluorescence traces of single molecules of a laser dye p-bis(o-methylstyryl)benzene (bis-MSB) and of a conjugated polymer polyfluorene.

Original languageEnglish (US)
Pages (from-to)234-235
Number of pages2
JournalChemistry Letters
Volume38
Issue number3
DOIs
StatePublished - Jul 20 2009

ASJC Scopus subject areas

  • Chemistry(all)

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