Single-cell vs. bulk activity properties of coastal bacterioplankton over an annual cycle in a temperate ecosystem

Xose Anxelu G. Moran, Alejandra Calvo-Díaz

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The connections between single-cell activity properties of heterotrophic planktonic bacteria and whole community metabolism are still poorly understood. Here, we show flow cytometry single-cell analysis of membrane-intact (live), high nucleic acid (HNA) content and actively respiring (CTC+) bacteria with samples collected monthly during 2006 in northern Spain coastal waters. Bulk activity was assessed by measuring 3H-Leucine incorporation and specific growth rates. Consistently, different single-cell relative abundances were found, with 60-100% for live, 30-84% for HNA and 0.2-12% for CTC+ cells. Leucine incorporation rates (2-153 pmol L-1 h-1), specific growth rates (0.01-0.29 day-1) and the total and relative abundances of the three single-cell groups showed marked seasonal patterns. Distinct depth distributions during summer stratification and different relations with temperature, chlorophyll and bacterial biovolume suggest the existence of different controlling factors on each single-cell property. Pooled leucine incorporation rates were similarly correlated with the abundance of all physiological groups, while specific growth rates were only substantially explained by the percentage of CTC+ cells. However, the ability to reduce CTC proved notably better than the other two single-cell properties at predicting bacterial bulk rates within seasons, suggesting a tight linkage between bacterial individual respiration and biomass production at the community level.

Original languageEnglish (US)
Pages (from-to)43-56
Number of pages14
JournalFEMS Microbiology Ecology
Volume67
Issue number1
DOIs
StatePublished - Jan 1 2009

Keywords

  • Atlantic
  • Bacterial activity
  • Bacterioplankton
  • Coastal
  • Seasonal variations
  • Single-cell properties

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Single-cell vs. bulk activity properties of coastal bacterioplankton over an annual cycle in a temperate ecosystem'. Together they form a unique fingerprint.

Cite this