Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Chen Liu, Michael Malek, Ivan Poon, Lanzhou Jiang, Arif M. Siddiquee, Colin J.R. Sheppard, Ann Roberts, Harry Quiney, Douguo Zhang, Xiaocong Yuan, Jiao Lin, Christian Depeursinge, Pierre Marquet, Shan Shan Kou

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.
Original languageEnglish (US)
Pages (from-to)1042
JournalPhotonics Research
Volume7
Issue number9
DOIs
StatePublished - Aug 14 2019

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