@article{6be14cd3e5844cd29fd47cb2bc2d8b5d,
title = "Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence",
abstract = "We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model.",
author = "Chen Liu and Michael Malek and Ivan Poon and Lanzhou Jiang and Siddiquee, {Arif M.} and Sheppard, {Colin J.R.} and Ann Roberts and Harry Quiney and Douguo Zhang and Xiaocong Yuan and Jiao Lin and Christian Depeursinge and Pierre Marquet and Kou, {Shan Shan}",
note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Australian Research Council (ARC) (DE120102352); National Natural Science Foundation of China (NSFC) (61427819); Shenzhen Science and Technology Innovation Commission (KQCS2015032416183980); Government of Guangdong Province (00201505); Schweizerischer Nationalfonds zur F{\"o}rderung der Wissenschaftlichen Forschung (SNF) (149652).",
year = "2019",
month = aug,
day = "14",
doi = "10.1364/PRJ.7.001042",
language = "English (US)",
volume = "7",
pages = "1042",
journal = "Photonics Research",
issn = "2327-9125",
publisher = "OSA Publishing",
number = "9",
}