Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms

Etienne Cuche, Pierre Marquet, Christian Depeursinge

Research output: Contribution to journalArticlepeer-review

1060 Scopus citations

Abstract

We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phasecontrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.

Original languageEnglish (US)
Pages (from-to)6994-7001
Number of pages8
JournalApplied Optics
Volume38
Issue number34
DOIs
StatePublished - Dec 1 1999

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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