Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions

M. Nei, T. Gojobori

Research output: Contribution to journalArticlepeer-review

3843 Scopus citations

Abstract

Two simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions are presented. Although they give no weights to different types of codon substitutions, these methods give essentially the same results as those obtained by Miyata and Yasunaga's and by Li et al.'s methods. Computer simulation indicates that estimates of synonymous substitutions obtained by the two methods are quite accurate unless the number of nucleotide substitutions per site is very large. It is shown that all available methods tend to give an underestimate of the number of nonsynonymous substitutions when the number is large.

Original languageEnglish (US)
Pages (from-to)418-426
Number of pages9
JournalMolecular Biology and Evolution
Volume3
Issue number5
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Genetics(clinical)

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