Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae

James E. Galagan*, Sarah E. Calvo, Christina Cuomo, Li Jun Ma, Jennifer R. Wortman, Serafim Batzoglou, Su In Lee, Meray Baştürkmen, Christina C. Spevak, John Clutterbuck, Vladimir Kapitonov, Jerzy Jurka, Claudio Scazzocchio, Mark Farman, Jonathan Butler, Seth Purcell, Steve Harris, Gerhard H. Braus, Oliver Draht, Silke BuschChristophe D'Enfert, Christiane Bouchier, Gustavo H. Goldman, Deborah Bell-Pedersen, Sam Griffiths-Jones, John H. Doonan, Jaehyuk Yu, Kay Vienken, Arnab Pain, Michael Freitag, Eric U. Selker, David B. Archer, Miguel Á Peñalva, Berl R. Oakley, Michelle Momany, Toshihiro Tanaka, Toshitaka Kumagai, Kiyoshi Asai, Masayuki Machida, William C. Nierman, David W. Denning, Mark Caddick, Michael Hynes, Mathieu Paoletti, Reinhard Fischer, Bruce Miller, Paul Dyer, Matthew S. Sachs, Stephen A. Osmani, Bruce W. Birren

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

955 Scopus citations

Abstract

The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious humaloven pathogen, and Aspergillus oryzae, used in the production of sake, miso and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence conservation revealed over 5,000 non-coding regions actively conserved across all three species. Within these regions, we identified potential functional elements including a previously uncharacterized TPP riboswitch and motifs suggesting regulation in filamentous fungi by Puf family genes. We further obtained comparative and experimental evidence indicating widespread translational regulation by upstream open reading frames. These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation.

Original languageEnglish (US)
Pages (from-to)1105-1115
Number of pages11
JournalNature
Volume438
Issue number7071
DOIs
StatePublished - Dec 22 2005

ASJC Scopus subject areas

  • General

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