Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces

David Sýkora, Frantisek Svec*, Jean Frechet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

136 Scopus citations

Abstract

Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic [pd(A)12-18] and oligothymidylic acids [d(pT)12-24] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalJournal of Chromatography A
Volume852
Issue number1
DOIs
StatePublished - Aug 6 1999

Keywords

  • Monolithic columns
  • Oligonucleotides
  • Stationary phases, LC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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