Semipreparative capillary electrochromatography

J. R. Chen, R. N. Zare*, E. C. Peters, F. Svec, Jean Frechet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Capillaries with inner diameters of 550 μm have successfully been packed with 1.5-μm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly-(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 μL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.

Original languageEnglish (US)
Pages (from-to)1987-1992
Number of pages6
JournalAnalytical Chemistry
Volume73
Issue number9
DOIs
StatePublished - May 1 2001

ASJC Scopus subject areas

  • Analytical Chemistry

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